Gespeichert in:
| Titel: | Methods in Cancer Stem Cell Biology |
|---|---|
| Von: |
by Said M. Afify, Masaharu Seno.
|
| Person: |
Afify, Said M.
author. aut http://id.loc.gov/vocabulary/relators/aut Seno, Masaharu. |
| Hauptverfassende: | , |
| Körperschaft: | |
| Format: | Elektronisch E-Book |
| Sprache: | Englisch |
| Veröffentlicht: |
Singapore :
Springer Nature Singapore : Imprint: Springer,
2023.
|
| Ausgabe: | 1st ed. 2023. |
| Schlagwörter: | |
| Medienzugang: | https://doi.org/10.1007/978-981-99-1331-2 |
| Zusammenfassung: | This book describes the use of stem cells and cancer stem cell generation in the inflammatory microenvironment (cancer-inducing niche) using induced pluripotent stem cells. It provides step-by-step techniques and manuals for studying stem cell and cancer stem cell generation with different applications in cancer research. The development of induced pluripotent stem cells has provided a new approach to studying cancer initiation by producing cancer stem cells without introducing mutations or foreign genes. The book features the research of the authors' group, which was the first to generate cancer stem cells from stem cells in the presence of inflammatory conditions. The 20 chapters if this book cover topics such as generating pluripotent stem cells, converting normal stem cells to cancer stem cells, enriching, isolating and evaluating cancer stem cells. Methods for evaluating the characteristics of cancer stem cells and possible therapies against them are also discussed. The book provides easy-to-follow protocols that help researchers in the study of cancer stem cells. Illustrations help readers understand how the method of cancer stem cell generation can be applied as an essential method for assessing the carcinogenic potential of various non-mutagenic compounds. It will be a useful resource for graduate students, researchers, technicians, and physicians working in academic, hospital, and pharmaceutical settings. . |
| Umfang: | 1 Online-Ressource (XVII, 243 p. 1 illus.) |
| ISBN: | 9789819913312 |
Internformat
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| 505 | 0 | |a 1. On the origin of cancer -- 2. Culture of Cells: Basic Principles -- 3. Stem cell culture from embryos -- 4. Reprogramming of normal cells -- 5. Maintenance of human Pluripotent Stem Cells -- 6. Identification of cancer stem cells by different molecular markers -- 7. Enrichment of Cancer Stem Cell from malignant tumor -- 8. Isolation of single clonal cell from primary cultured cells and establishment of a cancer stem cell line -- 9. Artificial Generation of Cancer Stem Cells from Normal Stem Cells -- 10. Quick method to assess non-mutagenic carcinogens with iPS cells -- 11. Self-renewal potential of caner stem cells -- 12. Differentiation potential of cancer stem cells in vitro -- 13. Tumor Angiogenesis by cancer stem cells in vivo -- 14. Invasion and Metastatic potential of cancer stem cells in vitro -- 15. Metastatic potential of cancer stem cells In Vivo- 16. Anchorage-independent cell growth assayfor Cancer Stem Cells: Tumorigenic assay in vitro -- 17. Tumorigenic potential of cancer stem cells in vivo -- 18. Development of immunoliposomes using monoclonal antibodies targeting cancer stem cells -- 19. In-vitro evaluation of anti-cancer stem cell drugs -- 20. In vitro Tumoroid Model Using Cancer Stem Cells. | |
| 520 | |a This book describes the use of stem cells and cancer stem cell generation in the inflammatory microenvironment (cancer-inducing niche) using induced pluripotent stem cells. It provides step-by-step techniques and manuals for studying stem cell and cancer stem cell generation with different applications in cancer research. The development of induced pluripotent stem cells has provided a new approach to studying cancer initiation by producing cancer stem cells without introducing mutations or foreign genes. The book features the research of the authors' group, which was the first to generate cancer stem cells from stem cells in the presence of inflammatory conditions. The 20 chapters if this book cover topics such as generating pluripotent stem cells, converting normal stem cells to cancer stem cells, enriching, isolating and evaluating cancer stem cells. Methods for evaluating the characteristics of cancer stem cells and possible therapies against them are also discussed. The book provides easy-to-follow protocols that help researchers in the study of cancer stem cells. Illustrations help readers understand how the method of cancer stem cell generation can be applied as an essential method for assessing the carcinogenic potential of various non-mutagenic compounds. It will be a useful resource for graduate students, researchers, technicians, and physicians working in academic, hospital, and pharmaceutical settings. . | ||
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| 650 | 2 | 4 | |a Biological Techniques. |
| 650 | 2 | 4 | |a Cancer Stem Cells. |
| 650 | 2 | 4 | |a Stem Cell Biology. |
| 700 | 1 | |a Seno, Masaharu. |e author. |4 aut |4 http://id.loc.gov/vocabulary/relators/aut | |
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| author | Afify, Said M. Seno, Masaharu |
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| contents | 1. On the origin of cancer -- 2. Culture of Cells: Basic Principles -- 3. Stem cell culture from embryos -- 4. Reprogramming of normal cells -- 5. Maintenance of human Pluripotent Stem Cells -- 6. Identification of cancer stem cells by different molecular markers -- 7. Enrichment of Cancer Stem Cell from malignant tumor -- 8. Isolation of single clonal cell from primary cultured cells and establishment of a cancer stem cell line -- 9. Artificial Generation of Cancer Stem Cells from Normal Stem Cells -- 10. Quick method to assess non-mutagenic carcinogens with iPS cells -- 11. Self-renewal potential of caner stem cells -- 12. Differentiation potential of cancer stem cells in vitro -- 13. Tumor Angiogenesis by cancer stem cells in vivo -- 14. Invasion and Metastatic potential of cancer stem cells in vitro -- 15. Metastatic potential of cancer stem cells In Vivo- 16. Anchorage-independent cell growth assayfor Cancer Stem Cells: Tumorigenic assay in vitro -- 17. Tumorigenic potential of cancer stem cells in vivo -- 18. Development of immunoliposomes using monoclonal antibodies targeting cancer stem cells -- 19. In-vitro evaluation of anti-cancer stem cell drugs -- 20. In vitro Tumoroid Model Using Cancer Stem Cells. |
| dewey-full | 571.978 616.994 |
| dewey-hundreds | 500 - Natural sciences and mathematics 600 - Technology (Applied sciences) |
| dewey-ones | 571 - Physiology & related subjects 616 - Diseases |
| dewey-raw | 571.978 616.994 |
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| dewey-sort | 3571.978 |
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| discipline | Biologie Medizin |
| edition | 1st ed. 2023. |
| format | Electronic eBook |
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| illustrated | Not Illustrated |
| indexdate | 2025-06-23T13:27:55Z |
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| isbn | 9789819913312 |
| language | English |
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| spelling | Afify, Said M. author. aut http://id.loc.gov/vocabulary/relators/aut Methods in Cancer Stem Cell Biology [electronic resource] / by Said M. Afify, Masaharu Seno. 1st ed. 2023. Singapore : Springer Nature Singapore : Imprint: Springer, 2023. 1 Online-Ressource (XVII, 243 p. 1 illus.) text txt rdacontent computer c rdamedia online resource cr rdacarrier text file PDF rda 1. On the origin of cancer -- 2. Culture of Cells: Basic Principles -- 3. Stem cell culture from embryos -- 4. Reprogramming of normal cells -- 5. Maintenance of human Pluripotent Stem Cells -- 6. Identification of cancer stem cells by different molecular markers -- 7. Enrichment of Cancer Stem Cell from malignant tumor -- 8. Isolation of single clonal cell from primary cultured cells and establishment of a cancer stem cell line -- 9. Artificial Generation of Cancer Stem Cells from Normal Stem Cells -- 10. Quick method to assess non-mutagenic carcinogens with iPS cells -- 11. Self-renewal potential of caner stem cells -- 12. Differentiation potential of cancer stem cells in vitro -- 13. Tumor Angiogenesis by cancer stem cells in vivo -- 14. Invasion and Metastatic potential of cancer stem cells in vitro -- 15. Metastatic potential of cancer stem cells In Vivo- 16. Anchorage-independent cell growth assayfor Cancer Stem Cells: Tumorigenic assay in vitro -- 17. Tumorigenic potential of cancer stem cells in vivo -- 18. Development of immunoliposomes using monoclonal antibodies targeting cancer stem cells -- 19. In-vitro evaluation of anti-cancer stem cell drugs -- 20. In vitro Tumoroid Model Using Cancer Stem Cells. This book describes the use of stem cells and cancer stem cell generation in the inflammatory microenvironment (cancer-inducing niche) using induced pluripotent stem cells. It provides step-by-step techniques and manuals for studying stem cell and cancer stem cell generation with different applications in cancer research. The development of induced pluripotent stem cells has provided a new approach to studying cancer initiation by producing cancer stem cells without introducing mutations or foreign genes. The book features the research of the authors' group, which was the first to generate cancer stem cells from stem cells in the presence of inflammatory conditions. The 20 chapters if this book cover topics such as generating pluripotent stem cells, converting normal stem cells to cancer stem cells, enriching, isolating and evaluating cancer stem cells. Methods for evaluating the characteristics of cancer stem cells and possible therapies against them are also discussed. The book provides easy-to-follow protocols that help researchers in the study of cancer stem cells. Illustrations help readers understand how the method of cancer stem cell generation can be applied as an essential method for assessing the carcinogenic potential of various non-mutagenic compounds. It will be a useful resource for graduate students, researchers, technicians, and physicians working in academic, hospital, and pharmaceutical settings. . Cancer. Biology Technique. Stem cells. Cancer Biology. Biological Techniques. Cancer Stem Cells. Stem Cell Biology. Seno, Masaharu. author. aut http://id.loc.gov/vocabulary/relators/aut SpringerLink (Online service) Springer Nature eBook Printed edition: 9789819913305 Printed edition: 9789819913329 Printed edition: 9789819913336 |
| spellingShingle | Afify, Said M. Seno, Masaharu Methods in Cancer Stem Cell Biology 1. On the origin of cancer -- 2. Culture of Cells: Basic Principles -- 3. Stem cell culture from embryos -- 4. Reprogramming of normal cells -- 5. Maintenance of human Pluripotent Stem Cells -- 6. Identification of cancer stem cells by different molecular markers -- 7. Enrichment of Cancer Stem Cell from malignant tumor -- 8. Isolation of single clonal cell from primary cultured cells and establishment of a cancer stem cell line -- 9. Artificial Generation of Cancer Stem Cells from Normal Stem Cells -- 10. Quick method to assess non-mutagenic carcinogens with iPS cells -- 11. Self-renewal potential of caner stem cells -- 12. Differentiation potential of cancer stem cells in vitro -- 13. Tumor Angiogenesis by cancer stem cells in vivo -- 14. Invasion and Metastatic potential of cancer stem cells in vitro -- 15. Metastatic potential of cancer stem cells In Vivo- 16. Anchorage-independent cell growth assayfor Cancer Stem Cells: Tumorigenic assay in vitro -- 17. Tumorigenic potential of cancer stem cells in vivo -- 18. Development of immunoliposomes using monoclonal antibodies targeting cancer stem cells -- 19. In-vitro evaluation of anti-cancer stem cell drugs -- 20. In vitro Tumoroid Model Using Cancer Stem Cells. Cancer. Biology Technique. Stem cells. Cancer Biology. Biological Techniques. Cancer Stem Cells. Stem Cell Biology. |
| title | Methods in Cancer Stem Cell Biology |
| title_auth | Methods in Cancer Stem Cell Biology |
| title_exact_search | Methods in Cancer Stem Cell Biology |
| title_full | Methods in Cancer Stem Cell Biology [electronic resource] / by Said M. Afify, Masaharu Seno. |
| title_fullStr | Methods in Cancer Stem Cell Biology [electronic resource] / by Said M. Afify, Masaharu Seno. |
| title_full_unstemmed | Methods in Cancer Stem Cell Biology [electronic resource] / by Said M. Afify, Masaharu Seno. |
| title_short | Methods in Cancer Stem Cell Biology |
| title_sort | methods in cancer stem cell biology |
| topic | Cancer. Biology Technique. Stem cells. Cancer Biology. Biological Techniques. Cancer Stem Cells. Stem Cell Biology. |
| topic_facet | Cancer. Biology Technique. Stem cells. Cancer Biology. Biological Techniques. Cancer Stem Cells. Stem Cell Biology. |
| work_keys_str_mv | AT afifysaidm methodsincancerstemcellbiology AT senomasaharu methodsincancerstemcellbiology AT springerlinkonlineservice methodsincancerstemcellbiology |